T4 ligase uniprot
WebT4 DNA Ligase 1–3u/µl/100u (Weiss units) $ 55.00 Your price: Log in T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´ …
T4 ligase uniprot
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WebNational Center for Biotechnology Information WebPerfect for multiple displacement amplification High fidelity - highly accurate replication Extreme processivity - capable of generating large fragments Isothermal amplification - no need for a thermocycler Reagents Supplied Need a custom/large volume order? Contact Us
WebFAQ. Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The … WebTaq DNA Ligase will ligate these substrates: Nicked DNA/RNA Isolated from a recombinant source Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction Taq DNA Ligase is NOT a substitute for T4 DNA Ligase Supplied with 10X Reaction Buffer containing NAD +
WebDNA ligase UniProt ID P00970 - DNLI_BPT4 Species Enterobacteria phage T4 (Bacteriophage T4) Gene 30 View Product On Supplier's Website Supplier provided … WebJun 14, 2024 · Our crystal structure of T4 DNA ligase bound to DNA shows a compact α-helical DNA-binding domain (DBD), nucleotidyl-transferase (NTase) domain, and OB-fold …
WebT4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates. Reagents Supplied
WebDec 2, 2024 · Canonical ligases, including bacteriophage T4 RNA ligase 1 or Saccharomyces cerevisiae tRNA ligase Trl1, are ATP-dependent enzymes that catalyze the formation of a 3′,5′-phosphodiester linkage between 3′-hydroxyl (3′-OH) and 5′-phosphate (5′-P) RNA termini ( Banerjee et al., 2024; El Omari et al., 2006; Greer et al., 1983; Gu et … banlangen keliWebThe T4-induced DNA ligase is partic- ularly interesting because of its unusual mode of joining action. T4 DNA ligase, which catalyzes the repair of phosphodiester breaks in DNA.DNA and DNA. RNA hybrid duplexes, is able to join duplex DNA molecules by two mechanisms. The enzyme can utilize either duplex DNA molecules which have pouille tennis rankingWebDec 31, 2024 · Group I chaperonins are a highly conserved family of essential proteins that self-assemble into molecular nanoboxes that mediate the folding of cytoplasmic proteins in bacteria and organelles. GroEL, the chaperonin of Escherichia coli, is the archetype of the family. Protein folding-independent functions have been described for numerous … pouf skin ottomanWebT4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ … poudre vihtavuori n340WebThe T4-induced DNA ligase is partic- ularly interesting because of its unusual mode of joining action. T4 DNA ligase, which catalyzes the repair of phosphodiester breaks in … banlieue trainingspak camoWebT4 DNA Ligase will ligate these substrates: dsDNA Nicked DNA/RNA Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids (1). poukama leirikeskusWebJul 15, 2024 · Circoviruses are the smallest single-stranded DNA viruses that infect mammalian species, avian species, fish, and insects. The infections of circoviruses are known to be associated with a series of fatal diseases, but the protease of circovirus still remains unknown. In this research, we identified viral capsid protein (Cap) as the … banky praha