site stats

T4 ligase uniprot

WebT4 DNA Ligase requires ATP as a cofactor. Highlights • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) • Fast—sticky … WebMar 1, 2011 · Plasmid pET16b-T4Dnl contains the gp30 gene which encodes for T4 DNA ligase. The target protein is fused with 10xHis tag to facilitate the purification and recovery. pET16b-T4Dnl was...

T4 DNA Ligase (5 U/µL) - Thermo Fisher Scientific

WebT4 DNA Polymerase is supplied in a buffer of 200 mM potassium phosphate (pH 6.5), 10 mM DTT and 50% glycerol. More Information Applications Generation of blunt double-stranded DNA from DNA containing 5'/3' overhangs 5'-end or 3'-end labeling of double-stranded DNA In vitro mutagenesis Buffer WebTotal vector size (bp) 6557 Vector type Bacterial Expression Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Low Copy Gene/Insert Gene/Insert name TS2126 RNA ligase Species Phage TS2126 Insert Size (bp) 1200 GenBank ID CQ796011.1 Promoter T7 Tag / Fusion Protein poudre vihtavuori n550 https://sptcpa.com

RCSB PDB - 1A0I: ATP-DEPENDENT DNA LIGASE FROM …

WebJun 10, 2013 · The T4 DNA ligase is the product of gene 30 ( lig) and it is essential for sealing Okazaki fragments during replication of the phage genome ( Miller et al., 2003 ). … WebT4 DNA Ligase from multiple suppliers was tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3’overhang or 5’ overhang containing oligonucleotides. The percent degradation by … Webgp4 is a hexameric protein containing two functional domains: helicase domain and primase domain. The helicase domain unwinds double-stranded DNA to provide template for replication. banky w campaign

National Center for Biotechnology Information

Category:T4 RNA Ligase 1 (ssRNA Ligase) NEB

Tags:T4 ligase uniprot

T4 ligase uniprot

Taq DNA Ligase NEB

WebT4 DNA Ligase 1–3u/µl/100u (Weiss units) $ 55.00 Your price: Log in T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´ …

T4 ligase uniprot

Did you know?

WebNational Center for Biotechnology Information WebPerfect for multiple displacement amplification High fidelity - highly accurate replication Extreme processivity - capable of generating large fragments Isothermal amplification - no need for a thermocycler Reagents Supplied Need a custom/large volume order? Contact Us

WebFAQ. Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The … WebTaq DNA Ligase will ligate these substrates: Nicked DNA/RNA Isolated from a recombinant source Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction Taq DNA Ligase is NOT a substitute for T4 DNA Ligase Supplied with 10X Reaction Buffer containing NAD +

WebDNA ligase UniProt ID P00970 - DNLI_BPT4 Species Enterobacteria phage T4 (Bacteriophage T4) Gene 30 View Product On Supplier's Website Supplier provided … WebJun 14, 2024 · Our crystal structure of T4 DNA ligase bound to DNA shows a compact α-helical DNA-binding domain (DBD), nucleotidyl-transferase (NTase) domain, and OB-fold …

WebT4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates. Reagents Supplied

WebDec 2, 2024 · Canonical ligases, including bacteriophage T4 RNA ligase 1 or Saccharomyces cerevisiae tRNA ligase Trl1, are ATP-dependent enzymes that catalyze the formation of a 3′,5′-phosphodiester linkage between 3′-hydroxyl (3′-OH) and 5′-phosphate (5′-P) RNA termini ( Banerjee et al., 2024; El Omari et al., 2006; Greer et al., 1983; Gu et … banlangen keliWebThe T4-induced DNA ligase is partic- ularly interesting because of its unusual mode of joining action. T4 DNA ligase, which catalyzes the repair of phosphodiester breaks in DNA.DNA and DNA. RNA hybrid duplexes, is able to join duplex DNA molecules by two mechanisms. The enzyme can utilize either duplex DNA molecules which have pouille tennis rankingWebDec 31, 2024 · Group I chaperonins are a highly conserved family of essential proteins that self-assemble into molecular nanoboxes that mediate the folding of cytoplasmic proteins in bacteria and organelles. GroEL, the chaperonin of Escherichia coli, is the archetype of the family. Protein folding-independent functions have been described for numerous … pouf skin ottomanWebT4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ … poudre vihtavuori n340WebThe T4-induced DNA ligase is partic- ularly interesting because of its unusual mode of joining action. T4 DNA ligase, which catalyzes the repair of phosphodiester breaks in … banlieue trainingspak camoWebT4 DNA Ligase will ligate these substrates: dsDNA Nicked DNA/RNA Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids (1). poukama leirikeskusWebJul 15, 2024 · Circoviruses are the smallest single-stranded DNA viruses that infect mammalian species, avian species, fish, and insects. The infections of circoviruses are known to be associated with a series of fatal diseases, but the protease of circovirus still remains unknown. In this research, we identified viral capsid protein (Cap) as the … banky praha