WebThere are many ways to clone small hairpin RNAs (shRNAs), and many of the. methods depend on the vectors you are using. Our lab often clones shRNA in pSicoR-derived … WebDescription of pSico and pSicoR vectors (53). A, section of pSico vector. In pSico, the U6 promoter that drives the expression of shRNA is made conditional in two steps. First, bifunctional...

Efficient delivery of lentiviral vectors into resting human CD4 T cells

WebOct 21, 2011 · shRNA-miRs-directed target knockdown is dependent on cell type and polymerase II used RNAi is often performed in cells that are relatively easy to grow and … WebA, analysis of L1 retrotransposition efficiency in HeLa cells transduced with lentiviruses carrying pSicoR-MS1 mCherry scrambled shRNA or shRNA against A3B mRNA and transfected with plasmid... hose hund m

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Webshort hairpin RNA (shRNA) expression. The former is based on a strategy in which the mouse U6 promoter has been modified by including a hybrid between a LoxP site and a TATA box. The ability ... (pSicoR) the 5 loxP site present in pLL3.7 was removed by digesting with XhoI and NotI and replaced with a diagnostic BamHI site by using the following Web丁香通商铺上海彩佑实业有限公司为您提供细胞库 / 细胞培养、试剂等分类产品85件，全方位满足您的科研实验采购需求 WebFIGURE 4 Inhibition of CD44 variants by CD44v6 siRNA and CD44v6 shRNA. A, Apc 10.1 cells were transfected with vector-neo or HAS2-neo and co-transfected with control scrsiRNA, or control pSicoR-scrshRNA, or CD44v6 siRNA, or pSicoR-CD44v6 shRNA plasmids. Transient transfection was carried out at 100 pmol of siRNA using Oligofectamine or 0.5 μg ... hose impulse test machine