Pcr-blunt topo
SpletRACE PCR products can be quickly and easily cloned using either the Zero Blunt TOPO PCR Cloning Kit for Sequencing (blunt-end PCR products) or the TOPO TA Cloning for Sequencing Kit (PCR products with 3′ A-overhangs). Using the protocols provided, the cDNA ends of rare (30 copies/cell) and long (9 kb) transcripts can be amplified and ... Splet27. okt. 2016 · Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique …
Pcr-blunt topo
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SpletpCR-BluntII-TOPO (Invitrogen, 450245) Zero Blunt TOPO PCR Cloning Kit. Map. Splet中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询 …
SpletTOPO PCR Cloning Kit (Cat. no. 450245) is shipped with only the Zero Blunt ™TOPO PCR Cloning reagents (Box 1). Box Store at 1 −30°C to −10°C in a non-frost-free freezer 2 … SpletpCR4Blunt-TOPO (linearized) Linearized vector with bound topoisomerase, for TOPO® cloning of blunt PCR products. Can be used to generate nested deletions for sequencing. …
Splet质粒载体网zlzt.com专业提供pCR-BluntII-TOPO-CDK8(human),产品编号为zl-007888,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹,质粒合成,质粒构建,质粒提取,质粒保藏! SpletZero Blunt™ PCR 克隆试剂盒有多种配置可供选择：含 One Shot™ TOPO10 化学感受态 大肠杆菌 （K2700-20 和 K2700-40）以及不含感受态细胞（K2750-20 和 K2750-40），有 20 次和 40 次反应试剂盒规格。 For Research Use Only. Not for use in diagnostic procedures. 规格 细菌或酵母菌株 TOP10 克隆方法 Blunt PCR 产品规格 试剂盒 产品线 Zero Blunt™ …
Splet5 min TA/Blunt-Zero Cloning Kit 基于拓扑异构酶的快速克隆试剂盒 TA克隆；平末端克隆 · 兼容：能够同时兼容TA克隆和平末端克隆； · 快速：加入片段，反应5 min即可。 · 高效：克隆效率高且阳性率＞95%。 1.兼容性强 2.简单快速 3.阳性率高：使用插入片段为1kb，转化后随即挑取单克隆进行菌落PCR鉴定，Marker为诺唯赞DL5000 DNA Marker（MD102） 本产 …
SpletAnalyze Sequence: pCR blunt II TOPO. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest … service urbanisme sanary sur merthe texas tenors ladySplet図5．コロニーダイレクトPCRの一例 0.5kbのPCRプロダクトをTAクローニングした後、図3に示した様々なプライ マーの組み合わせを用いてコロニーダイレクトPCRを行いました。 （R1はベ クター上に設計したリバースプライマー） M：100bpラダーマーカー 1～5：各 ... service user feedback learning disabilitiesSpletpCR-Blunt载体质粒图谱、序列、价格、抗性、测序引物、大小等基本信息_生物风载体 pCR-Blunt 价格： 1500元 联系方式： I47-825O-882O 相关技术服务： 质粒构建 基因合成 质粒大提 立刻购买 买家导航 载体基本信息 载体质粒图谱和多克隆位点信息 载体简介 载体序列 service user groups nhsSpletpCR4-TOPO Parental sequencing vector for TOPO® TA cloning of PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download … service user care plan templateSpletZero Blunt® ® TOPO® PCR Cloning Reagents Zero Blunt TOPO® PCR Cloning reagents (Box 1) are listed below. Note that the user must supply the proofreading polymerase. Store Box 1 at -20°C. Item Concentration Amount pCR ®-Blunt II-TOPO 20 10 ng/µl plasmid DNA in: 50% glycerol 50 mM Tris-HCl, pH 7.4 (at 25°C) 1 mM EDTA 2 mM DTT 0.1% Triton ... service user groups in health and social careSpletAdd A-tailed PCR products of DNA polymerase (such as Taq Vazyme's Taq series) into 5 × Ultra-Universal TOPO Cloning Mix, then react at 20 ~ 37°C for 5 min. Blunting factor in Mix removes the adenine at the end of the amplifi - cation product to form blunt-end products. The 5'-OH of blunt-end products attack the phosphate bond the texas tenors unchained melody