WebHost cells produce energy-rich nutrients via catabolism (through autophagy, mitophagy, and aerobic glycolysis), which are then transferred to cancer cells to fuel anabolic tumor growth. Stromal cell-derived L-lactate is taken up by cancer cells and is used for mitochondrial oxidative phosphorylation (OXPHOS) to produce ATP efficiently. WebMitochondrial membrane potential was measured in wild-type or HILPDA knockout Huh7 cells using MitoTracker Red CMXRos (Invitrogen, M7512) according to the manufacturer ... and incubated in normoxia or hypoxia for 72 hr. Then, cells were fixed in 3.7% formaldehyde (Electron Microscopy Sciences, 15710-S) for 15 min at room temperature …
A Reproducible, Objective Method Using MitoTracker® …
WebAs a relic of ancient bacterial endosymbionts, mitochondria play a central role in cell metabolism, apoptosis, autophagy, and other processes. However, the function of mitochondria-derived nucleic acids in cellular signal transduction has not … WebNotably, by using Atto 647N as a live-cell mitochondrial marker, we obtain 2.5-time enhancement of brightness and photostability likened from the most commonly used SiR color in long-term imaging. Our strategy has enlarged one scientist's toolkit for understanding the dynamics and interactions of subcellular structures in living printable. scientific notation worksheets with example
Store at -20°C MitoTracker Green FM - Cell Signaling Technology
WebMitotracker is provided with 50 μg tubes and should can stored in the darks at -20oxygenC, indefinitely; it is stable for solution for up to 1 month, though ourselves have don tested its effectiveness following solubilisation and storage Web7 nov. 2005 · Cells were treated with 10 μg/ml CCN1 for 6 h, stained with MitoTracker for 5 min, fixed, immunostained for either Bax or cytochrome c, and counterstained with DAPI. Arrows point to apoptotic cells. Bar, 10 μm. Web11 apr. 2024 · At the end of the experiment, KGN cells were washed with PBS and fixed with 4% PFA. Then, KGN cells were incubated with β-Gal substrate at 37 °C overnight. … praxis balance bramsche