Egfp-mcherry-lc3b
WebDownload scientific diagram RNAi knockdown of VcP impairs autophagosome maturation. (A) mcherry-eGFP-Lc3b stable MeFs transfected with nontargeting, Rab7 or VcP siRNA. scale bars equal 10 µm. WebJul 14, 2024 · Autophagy inhibitor 3-methyladenine, EGFP-LC3 and mCherry-GFP-LC3B plasmid transfection were used for further study. Results: Metformin inhibited significantly the viability of MHCC97H and HepG2 cells in a dose- and time-dependent manner.
Egfp-mcherry-lc3b
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WebPmCherry-EGFP-LC3b is a double fluorescent reporter plasmid for human mammalian cell autophagy. pmCherry-EGFP-LC3b Reference Foot-and-mouth disease virus capsid … WebPlasmid pmCherry-EGFP from Dr. Ivan Yudushkin's lab contains the insert mCherry-EGFP and is published in Mol Cell. 2024 Feb 2;65(3):416-431.e6. doi: …
WebApr 13, 2024 · (G) Representation of the GFP–SEPT6, K63 polyubiquitin and mCherry-LC3B microdomains of the septin cage from E. 1 and 2 mark the beginning and end of the dashed white line from F. Scale bar: 1 µm. (H) Fluorescence intensity profiles of GFP–SEPT6, K63 polyubiquitin and mCherry-LC3B across the dashed white line shown … WebNov 7, 2015 · Plasmids construction pQCXIP-tfLC3B expressing mCherry-EGFP tandem fluorescent- tagged LC3B follows.First, mCherry gene amplifiedfrom pmCherry-C1 (Clontech, CA, USA) tem-plate PCR(up primer: -GTCGCGGCCGCCACCATGGTGAGCAA-GGGCGAGGAG-3 downprimer: -GCTGGATCCACCTCCGCTTCCA …
WebMcherry Egfp Lc3 Lentivirus Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... 16HBE cells were treated with TGF-β3 in the presence of 3-MA (h) or Baf A1 (j). Then, the expression of LC3B and SQSTM1 was … WebFeb 28, 2024 · MDFs stably infected with pBABE-puro/mCherry-EGFP-LC3B were seeded in eight-well chamber µ-slides (Ibidi) and treated as indicated. On harvest, cells were fixed with 4% PFA in PBS for 10 min, washed with PBS, and stained with DAPI (ThermoFisher) for 10 min. For live-cell imaging, MDFs were seeded in a 35 mm µ-Dish (Ibidi).
Web本发明公开了去铁酮在抑制氨基糖苷类药物的耳毒性中的应用，属于药物性耳聋领域。本发明公开了去铁酮(DFP)在保护氨基糖苷类药物引起的听力损失中的应用。所述的DFP是唯一口服有活性的铁螯合剂，临床用于治疗地中海贫血中的输血性铁过载。本发明首先研究了氨基糖苷类药物损伤，类毛细胞HEI ...
Webmcherry-GFP-Lc3 reporter cells enable flow cytometric quantification and sorting of cells based on autophagic flux. (A) Quantification of autophagic flux by flow cytometry using reporter cells... drs in casualtyWebAug 12, 2016 · A couple who say that a company has registered their home as the position of more than 600 million IP addresses are suing the company for $75,000. James and … coloring books kids freeWebFinally, when PC3 cells expressing mCherry-eGFP- LC3B (Fig. 6A,B) or PC3 cells transfected with eGFP-p62 (Fig. 6C,D) were treated with GDC-0941 +/2 CQ with spiked-in LynxTag-CQ-blue, the ... dr sinatra lung and bronchial support reviewsWebAug 24, 2024 · We found that GFP-LC3B is recruited to mCherry-ATL2 before the trafficking of both proteins to BFP-mLAMP1–tagged lysosomes during starvation (Fig. S4 C). Overall, our data suggest that ATL2 is not degraded by the proteasome-mediated ER-associated degradation pathway, and is instead lysosomally degraded during starvation. coloring books of birdsWebOct 21, 2024 · For analysis of mCherry-EGFP-LC3b and mCherry-EGFP-MLS cells, red-only structures were determined by weighting the red signal to match the green signal and by dividing the weighted red signal by ... dr sincenyWebThe City of Fawn Creek is located in the State of Kansas. Find directions to Fawn Creek, browse local businesses, landmarks, get current traffic estimates, road conditions, and … coloring books from photosWebGFP-LC3单荧光和mCherry-GFP-LC3双荧光指示系统. 自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点。当自噬溶酶体形成后，酸性环境使GFP荧光淬灭，而mCherry荧光不受影响，自噬溶酶体呈现红色荧光（图7）。 dr sincavage ob/gyn