Clonogenic efficiency
is an in vitrocell survival assay. It assesses the ability of single cells to survive and reproduce to form colonies1. This assay was first described in the 1950s, where it was used to study the effects of radiation on cancer cell survival and growth and has subsequently played an essential role in radiobiology2. In order to … See more Clonogenic assays are widely used in the field of cancer research as the formation of clones is interpreted as a trait of cancer cells with tumor-initiating capabilities. While this assay was initially used in the field of radiobiology, … See more The information in this section is adapted from Franken et al. (2006) who published a clonogenic assay protocol inNature Protocols1, combined with some insights from my own … See more It really is as simple as manually counting your colonies for each treatment condition and representing the data as a survival curve (you should … See more Clonogenic assays are typically performed in 6-well or 24-well plates. Cells need to be sparsely and evenly plated so isolated coloniescan form. … See more
Clonogenic efficiency
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WebIn this study, we characterized the self-renewal capability, multi-lineage differentiation capacity, and clonogenic efficiency of human dental pulp stem cells (DPSCs). DPSCs were capable of forming ectopic dentin and associated pulp tissue in vivo. Stromal-like cells were reestablished in culture from primary DPSC transplants and re ... WebOGG1 expression was monitored by RT-qPCR analysis and immunoblotting. (B) Cells were submitted to irradiation and their clonogenic efficiency was determined. Cloning efficiency of unexposed cells was set at 100%. Results of triplicate experiments are shown.
WebCombined treatment with Dox-NP further enhanced therapeutic efficiency in metastatic resistant BRCA wt cell lines. The clonogenic survival was significantly reduced after treatment with [125 I]-PARPi-01 in all TNBC lines investigated. Therapeutic efficacy was further enhanced after combined treatment with chemotherapeutics. WebApr 11, 2024 · Resistance to neoadjuvant chemoradiation therapy is a significant clinical challenge in the management of rectal cancer. There is an unmet need to identify the underlying mechanisms of treatment resistance to enable the development of biomarkers predictive of response and novel treatment strategies to improve therapeutic response. …
WebJul 5, 2016 · It is very much important to optimize the cell numbers in the Clonogenic assay, because larger number of cell leads to the merger of the colonies and the … WebNational Center for Biotechnology Information
WebQuantitative (TNC and total CD34+cells) and qualitative variables [viable CD45+ (vCD45+), vCD34+ and clonogenic efficiency [(CLONE), quotient of post-thaw colony-forming units (CFU)] and pre-freeze CD34+ cells predicted engraftment in univariate analysis however, only 2 qualitative variables remained significant in the multivariate analysis.
WebApr 8, 2024 · In the clonogenic assay, cells were suspended and plated at a density of 600 cells per 34.8 mm dish. ... H–J The knockdown efficiency of PLCG2 in H1299 after PLCG2 siRNA transfection by qPCR and ... naira creationsWebMar 13, 2011 · The clonogenic (or colony forming) assay has been established for more than 50 years; the original paper describing the technique was published in 1956. ... our … medley of musicalsWebApr 8, 2024 · In the clonogenic assay, cells were suspended and plated at a density of 600 cells per 34.8 mm dish. Two weeks later, all formed colonies were stained with 0.05% crystal violet for 30 min. The assays were performed in duplicate plates, and a total of nine fields were counted for each plate. ... H–J The knockdown efficiency of PLCG2 in H1299 ... nair aftercareWebOct 25, 2024 · The growing colonies eventually became self sustaining, and clonogenic growth with nearly 100% plating efficiency (PE), defined as the number of clonogenic … naira exchange todayWebApr 15, 2003 · The loss of membrane integrity after 1 hour of treatment correlated strongly with reduced clonogenic efficiency. However, at very low concentrations of granzymes, 7-AAD staining underestimated the loss of clonogenic efficiency, perhaps because some 7-AAD low cells were committed to die, but had not yet experienced membrane changes. nair ajith pWebNational Center for Biotechnology Information medley of rice and ancient grainsWebOct 29, 2024 · The clonogenic assay is a versatile and frequently used tool to quantify reproductive cell survival in vitro. Current state-of-the-art analysis relies on plating … naira exchange for dollar in the black market